In the middle of August 2013, photosynthesis of population was measured, and plant species: red sand. The group photosynthesis measurement system consists of li-8100 closed-circuit soil carbon flux automatic measurement system (li-cor, USA) and assimilation box designed and manufactured by Beijing ligotai science and Technology Co., Ltd. li-8100 is an instrument produced by li-cor company of USA for soil carbon flux measurement. The concentration of CO2 and H2O is measured by infrared gas analyzer. The length, width and height of assimilation boxes were all 50 cm. The assimilator is controlled by li-8100. After the measurement parameters are set, the instrument can run automatically.
SU Peixi
A small lysimeter was made by ourselves, which simulated the natural conditions and selected typical desert plants as the object to study the water consumption and its law. Repeat 3 times for each plant. In 2011, the experiment of physiological water demand and water consumption of desert plants was carried out with the soil water content kept at (50 ± 10)% of the field water capacity; in 2012, the experiment of physiological water demand and water consumption was carried out with the soil water content kept at (20 ± 5)% of the field water capacity under stress.
SU Peixi
A small lysimeter was made by ourselves, which simulated the natural conditions and selected typical desert plants as the object to study the water consumption and its law. Repeat 3 times for each plant.
SU Peixi
In July and mid August 2012, plant species: Caragana. Using Li-6400 portable photosynthesis system (li-cor, USA) and li-3100 leaf area meter, the photosynthetic physiological characteristics of desert plants were observed. The symbols in the observation data have the following meanings: Obs, number of observations;Photo, net photosynthetic rate, moles of CO2 times m minus 2 times s minus 1; Cond, stomatal conductance, mol H2O•m -- 2•s -- 1;Ci, intercellular CO2 concentration, moles of CO2 times mol-1; Trmmol, transpiration rate, mmol H2O•m -- 2•s -- 1;Vpdl, water vapor pressure deficit, kPa; Area, leaf Area, cm2;Tair, atmospheric temperature, ℃; Tleaf, leaf surface temperature, ℃;CO2R, CO2 concentration in the reference chamber, moles of CO2•mol-1; CO2S, sample chamber CO2 concentration, moles of CO2•mol-1;H2OR, water in the reference chamber, mmol H2O•mol-1; H2OS, sample chamber moisture, mmol H2O•mol-1;PARo, photon flux density, mole •m -- 2•s -- 1; Rh-r, reference room air relative humidity, %;Rh-s, relative humidity of air in sample room, %; PARi, photosynthetic effective radiation, moles •m -- 2•s -- 1;Press, atmospheric pressure, kPa; Others are the state parameters of the instrument at the time of measurement.
SU Peixi
The hydrological monitoring of Picea crassifolia and main shrub vegetation types, including canopy interception, soil moisture content and stemflow, was carried out at different altitude gradients in Pailugou catchment of Qilian Mountain. The monitoring time was the dynamic monitoring of growth season in 2012 and 2013.
LIU Xiande
In the middle of July, 2011, 1. Elaeagnus angustifolia, 2. Blister. Using Li-6400 portable photosynthesis system (li-cor, USA) and li-3100 leaf area meter, the photosynthetic physiological characteristics of desert plants were observed. The symbols in the observation data have the following meanings: Obs, number of observations;Photo, net photosynthetic rate, moles of CO2 times m minus 2 times s minus 1; Cond, stomatal conductance, mol H2O•m -- 2•s -- 1;Ci, intercellular CO2 concentration, moles of CO2 times mol-1; Trmmol, transpiration rate, mmol H2O•m -- 2•s -- 1;Vpdl, water vapor pressure deficit, kPa; Area, leaf Area, cm2;Tair, atmospheric temperature, ℃; Tleaf, leaf surface temperature, ℃;CO2R, CO2 concentration in the reference chamber, moles of CO2•mol-1; CO2S, sample chamber CO2 concentration, moles of CO2•mol-1;H2OR, water in the reference chamber, mmol H2O•mol-1; H2OS, sample chamber moisture, mmol H2O•mol-1;PARo, photon flux density, mole •m -- 2•s -- 1; Rh-r, reference room air relative humidity, %;Rh-s, relative humidity of air in sample room, %; PARi, photosynthetic effective radiation, moles •m -- 2•s -- 1;Press, atmospheric pressure, kPa; Others are the state parameters of the instrument at the time of measurement.
SU Peixi
A small lysimeter was made to simulate the natural conditions and select typical desert plants as the objects to study the water consumption of drought stress treatment. Repeat 3 times for each plant. In 2012, the soil water content was kept at (20 ± 5)% of the field water capacity, and experiments on physiological water demand and water consumption were carried out under stress. In 2013, the soil water content was kept at (10 ± 3)% of the field water capacity, and further experiments on water consumption and water consumption law were carried out under drought stress.
SU Peixi
The leaf area of five typical species of jinjier, jilialu, jinlumei, huangxiaoba and Ganqing jinjier in Dayekou watershed of Qilian Mountain was measured by LAI-2200 canopy analyzer.
LIU Xiande
In this project, Ammopiptanthus mongolicus, a typical desert plant, is taken as the research object. Through optimizing the protein extraction and purification system of Ammopiptanthus mongolicus, IEF and 2-D two-dimensional electrophoresis techniques are used to obtain soluble protein electrophoresis maps of Ammopiptanthus mongolicus, and protein spots differentially expressed under drought stress are analyzed and obtained, which provides technical guarantee for subsequent mass spectrometry to identify protein functions and construct Ammopiptanthus mongolicus water stress response network.
SU Yanhua
Leaf area index, also known as leaf area coefficient, refers to the multiple of the total area of plant leaves in the land area per unit land area. Leaf area index is an important structural parameter of ecosystem, which is used to reflect the number of plant leaves, the change of canopy structure, the life activity of plant community and its environmental effect, to provide structured quantitative information for the description of material and energy exchange on the canopy surface, and to balance the energy of carbon accumulation, vegetation productivity and the interaction between soil, plant and atmosphere, Vegetation remote sensing plays an important role. Plant canopy imager CI - 110 was used to measure the alpine shrub and spruce leaf area index in hulugou watershed. The measurement period is July 22, 2014. It includes the main shrub types and Picea crassifolia forest in hulugou watershed. The data set mainly includes the original data of CI-110 measurement, including image and leaf area analysis image.
LIU Zhangwen, SONG Yaoxuan
This data set is the surface evapotranspiration data of Four Typical Shrub Communities in hulugou watershed. The observation period is from July 16 to August 23, 2013, which is the daily scale data. The data content includes precipitation data, evaporation and infiltration data observed by lysimeter. The data set can be used to analyze the evapotranspiration data of alpine shrub and forest. Data quality information: data quality is high, daily evapotranspiration data observation is complete. Data source description: a small lysimeter with an inner diameter of 25 cm and a depth of 30 cm was selected for evapotranspiration under the canopy. Two lysimeters were set up in each sample plot of evapotranspiration under the Bush, and one lysimeter was set up for each kind of Bush in the transplanting experiment. The undisturbed undisturbed soil column with the same height as the barrel shall be placed in the inner barrel during the layout, and the outer barrel shall be buried in the soil. During the embedding, the outer barrel shall be 0.5-1.0 cm higher than the ground, and the outer edge of the inner barrel shall be designed with a 2.0 cm wide rain shield to prevent the surface runoff from entering the lysimeter. Lysimeter was set up in the nearby meteorological station to measure the evapotranspiration of grassland, and a small evapotranspiration meter with an inner diameter of 25 cm and a depth of 30 cm was set up in the Picea koraiensis forest sample plot to measure the evaporation under the forest. All lysimeters shall be weighed on time at 20:00 every day (electronic balance sensing capacity is 1.0 g, which is equivalent to 0.013 mm evaporation). During observation, windproof treatment shall be done to ensure the accuracy of measurement. Data processing method: evapotranspiration is mainly calculated by mass conservation in lysimeter method. According to lysimeter design principle, evapotranspiration is mainly determined by mass difference in two consecutive days. Because it is weighed every day, it is calculated by water balance.
SONG Yaoxuan, LIU Zhangwen
This data set is the precipitation characteristic data in the precipitation interception data of alpine shrub in hulugou basin in the upper reaches of Heihe River in 2012. The observation date is from October 2, 2011 to September 24, 2012. The observation contents include precipitation, precipitation duration, precipitation intensity and frequency of throughfall. The observation data are recorded by self recording rain gauge and artificial rain gauge.
SONG Yaoxuan, LIU Zhangwen
Vegetation functional type (PFT) is the combination of large plant species according to the ecosystem function of plant species and the way of resource utilization. Each plant functional type shares similar plant properties, which simplifies the diversity of plant species to the diversity of plant function and structure.Vegetation functional types have been used in the dynamic global vegetation model (DGVM) to predict changes in ecosystem structure and function under global change scenarios.The 1km vegetation functional pattern map of heihe basin is based on the 1km land cover map of heihe basin (MICLCover subset of heihe basin), and is divided by using the vegetation functional climate rules proposed by Bonan et al. (2002).The climate data utilize the 0.1 degree atmospheric drive data of he jie and Yang kun, developing China region from 1981 to 2008.This map can be used in the land surface process model of heihe river basin.
RAN Youhua
It is of great significance to carry out the quantitative study on the evapotranspiration of forest vegetation in Qilian Mountain, to correctly understand the hydrological function of the forest ecosystem in Qilian Mountain, to understand the water cycle process and to develop the hydrological model of the watershed, and to make a reasonable forest management plan. Forest evapotranspiration is mainly composed of soil surface evaporation, vegetation transpiration and canopy interception water evaporation. Traditional evapotranspiration research methods can be divided into two categories: actual measurement and estimation. The actual measurement methods include hydrology method, micro meteorology method and plant physiology method; the estimation method is to calculate Evapotranspiration by model, mainly including analysis model and empirical model. However, none of these methods can effectively distinguish forest transpiration from evaporation. The trunk liquid flow method can effectively calculate the transpiration of forest land by measuring the transpiration water consumption of trees. The trunk liquid flow method can effectively calculate the transpiration of forest land by measuring the transpiration water consumption of trees. The transpiration water consumption of Picea crassifolia forest was measured by thermal pulse technique, and the scale was extended to the stand scale to indicate the transpiration water consumption of Picea crassifolia forest.
CHANG Xuexiang
The evapotranspiration and soil evapotranspiration of lycium rubra and red sand of small shrubs in typical desert weather were observed by using infrared gas analyzer to measure water vapor flux. The measurement system consists of li-8100 closed-circuit automatic measurement of soil carbon flux (li-cor, USA) and an assimilation box designed and manufactured by Beijing ligotai technology co., LTD. Li-8100 is an instrument produced by li-cor for soil carbon flux measurement. It USES an infrared gas analyzer to measure the concentration of CO2 and H2O.The length, width and height of the assimilation box are all 50cm.The assimilation box is controlled by li-8100. After setting up the measurement parameters, the instrument can run automatically.
SU Peixi
As determined in mid-august 2013, planting species: bubbly spines (different habitats are mid-range intermountain lowland and gobi), red sand (different habitats are mid-range gobi and downstream gobi). Using the brother company of LI - 6400 Portable Photosynthesis System (Portable Photosynthesis System, LI - COR, USA) and LI - 3100 leaf area meter, etc., to the desert plant photosynthetic physiological characteristics were observed. The symbolic meaning of the observed data is as follows: Obs，observation frequency ； Photo ，net photosynthetic rate，μmol CO2•m–2•s–1； Cond stomatal conductance，mol H2O•m–2•s–1 ; Ci, Intercellular CO2 concentration, μmol CO2•mol-1； Trmmol，transpiration rate，mmol H2O•m–2•s–1； Vpdl，Vapor pressure deficit，kPa； Area，leaf area，cm2； Tair，free air temperature ，℃； Tleaf，Leaf temperature，℃； CO2R，Reference chamber CO2 concentration，μmol CO2•mol-1； CO2S，Sample chamber CO2 concentration，μmol CO2•mol-1； H2OR，Reference chamber moisture，mmol H2O•mol-1； H2OS，Sample chamber moisture，mmol H2O•mol-1； PARo，photon flux density，μmol•m–2•s–1； RH-R，Reference room air relative humidity，%； RH-S，Relative humidity of air in sample room，%； PARi，Photosynthetic effective radiation，μmol•m–2•s–1； Press，barometric pressure，kPa； Others are the state parameters of the instrument at the time of measurement.
SU Peixi
Sponsored by the European commission VEGETATION sensors in March 1998 by SPOT - 4 was deployed, from April 1998 to receive SPOTVGT for global VEGETATION observation data, the data by the Swedish Kiruna ground station is responsible for receiving, the image quality monitoring center in Toulouse, France is responsible for the image quality and provide the related parameters (e.g., scaling),Eventually, Belgium's Flemish Institute for Technological Research (Vito) 's VEGETATION processing Centre (CTIV) was responsible for pre-processing the data into 1km of daily global data.Preprocessing includes atmospheric correction, radiometric correction, and geometric correction to produce the maximum synthesis of NDVI data in 10 days, and set the value from -1 to -0.1 to -0.1, and then convert to the DN value of 0-250 through the formula DN= (NDVI+0.1)/0.004. This data set is mainly for normalized vegetation index (NDVI) of the qaidam river basin in the long time series, including spectral reflectance of four bands synthesized every 10 days from 1998 to 2008 and maximum NDVI in 10 days. The spatial resolution is 1km and the temporal resolution is 10 days.File formats :.hfr and.img.The file naming rule is CHN_NDV_YYYYMMDD, where YYYYMMDD is the date of the day that the file represents and is the main identifier that distinguishes it from other files.Remote sensing image files with suffixes.img and.hdf, which are used by users to analyze vegetation index, can be opened in ENVI and ERDAS software
Greet Janssens, Flemish Institute for Technological Research (VITO)
Background: this data interchange is the first data interchange of the key project of "integrated study of eco-hydrological processes in heihe basin", "genomics research on drought tolerance mechanism of typical desert plants in heihe basin".The main research targets of the key projects is a typical sand desert plants are Holly, using the current international advanced a new generation of gene sequencing technology to the whole genome sequence and gene transcription of Holly group sequence decoding, so as to explore related to drought resistance gene and gene groups, and transgenic technology in model to verify their drought resistance in plants. Process and content: as genome sequencing requires special sequencing equipment, the project is huge and the process is complex (mainly including genome library construction, sequencing, data analysis and genome assembly), so it needs to be completed by a professional sequencing company.After contacting with sequencing companies, we learned that before sequencing an unknown genome, the size and complexity of the genome should be predicted, which is a necessary prerequisite for designing sequencing schemes and strategies.Therefore, in 2013, we mainly predicted the chromosome composition, genome size and complexity of sand Holly, and successfully established the extraction and purification method of its genomic DNA.The results showed that the plant was diploid, the genome was composed of 9 staining lines (18 lines of diploid), and the genome size was 1.07G.The quality test results of the genomic DNA indicated that the requirements of the obtained DNA complex sequencing have been sent to the sequencing company for library construction and sequencing, which is now in progress.In addition, in order to obtain a large number of uniform plant materials, we have discussed the induction of callus, which has been successful.Due to these reasons, we were unable to complete the genome sequencing and submit the relevant data of sand Holly in accordance with the original plan of the project this year, mainly because we did not count the predicted contents of the genome before. Data usage: the data obtained in this year on ploidy, karyotype composition and genome size of lycopodium SPP.The success of the callus induction provides a high-quality material guarantee for the subsequent transcriptome sequencing and drought-resistance mechanism research experiments, and it is also a new contribution to the cytological and physiological research of the plant.
HE Junxian, GU Lifei
1. Data overview The data set of the base camp integrated environmental observation system is a set of ENVIS (IMKO, Germany) which was installed at the base camp observation point by qilian station.It is stored automatically by ENVIS data mining system. 2. Data content This data set is the scale data from January 1, 2012 to December 31, 2012.Including air temperature 1.5m, humidity 1.5m, air temperature 2.5m, humidity 2.5m, soil moisture 0cm, precipitation, wind speed 1.5m, wind speed 2.5m, wind direction 1.5m, geothermal flux 5cm, total radiation, surface temperature, ground temperature 20cm, ground temperature 40cm, ground temperature 60cm, ground temperature 80cm, ground temperature 120cm, ground temperature 160cm, CO2, air pressure. 3. Space and time scope Geographical coordinates: longitude: 99° 53’e;Latitude: 38°16 'N;Height: 2980.2 m
SI Jianhua
In mid-july 2011, photosynthetic organs (leaves or assimilating branches) of typical desert plants were collected and brought back to the laboratory in a liquid nitrogen tank for determination. The analysis indexes mainly include soluble protein unit: mg/g;Free amino acid unit: g/g;Chlorophyll content unit: mg/g;Superoxide dismutase (SOD) unit: U/g FW;Catalase (CAT) unit: U/(g•min);POD unit: U/(g•min);Proline (Pro) unit: g/g; Soluble sugar unit: g/g;Malondialdehyde (MDA) is given in moles per liter.
SU Peixi
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